During backup, as long as there are files that need to be backed up but are locked at the same time, you cannot delete the files that need to be cleared.
Directory file description (now C:\SOURCE and C:\DESTINATION are exactly the same)
C:\SOURCE
C:\DESTINATION
aaa.docx
=
aaa.docx
bbb.xls
=
bbb.xls
lock.txt
=
lock.txt
Modify files under SOURCE
2.1 Modify the contents of C:\SOURCE\lock.txt file
2.2 Modify the file name of C:\SOURCE\bbb.xls to ccc.xls
Note that files in the destination won't be deleted if there were any errors at any point.
In general it is not good idea to try to operate on opened/locked files. Does not matter if rclone or any other software. End result might be unpredictable.
I would strongly advice to use filesystem snapshots (VSS on Windows) in such situations. For backups IMO it is a must approach.
Here you are good summary written by @asdffdsa how to use it: