why not run a quick test?
tho, for fun, i guess that rclone will transfer the file successfully.
as rclone has two caches, and the cache you are asking about is the dir cache, not the file cache.
might check out my comparison of the two caches.
https://forum.rclone.org/t/status-about-using-rclone-for-music-storage-playback-in-2021-access-times-improved/27648/34